#3357 MORPHOMETRIC AND FUNCTIONAL EVALUATION OF A NON-RENAL VASCULATURE IN FEMALE AND MALE PKD/MHM (CY/+) RATS

Abstract Background and Aims Polycystic kidney disease (PKD) is a cystic genetic disorder of the kidneys. Vascular abnormalities are most important non-cystic complications in PKD. In present study, we evaluated aortic morphometry vascular function female male PKD/Mhm (Cy/+) rats, an animal model autosomal dominant Method Thoracic rings from six month-old heterozygous (n = 10; 307±4g) 470±24g) age-matched non-affected homozygous (+/+) Control-female 9; 312±6g) Control-male 13; 460±14g) rats were used a) to evaluate both contractile/relaxation responses ex vivo using organ bath experiments b) study assessed by hematoxylin&eosin staining. Furthermore, immunohistochemistry for cleaved poly (ADP-ribose) polymerase (PARP)-1 was performed. Results Although there no significant difference body weight, serum creatinine levels increased PKD-female PKD-male compared with respective controls (PKD-female 50.0±1.7 vs. 43.9±2.1 µmol/L; 178.7±21.0 40.4± 1.3 µmol/L, p<0.05) found be higher than PKD-females (p<0.0001). Compared Control-male, showed decreased maximum relaxation acetylcholine (55±2% 77±1%, p<0.05), indicating impaired endothelial function. Contractile phenylephrine (3.0±0.1g 3.4±0.1g, high potassium (3.3±0.1g 3.7±0.1g, reduced Control-male. Additionally, aortas, concentration-response curve sodium nitroprusside, endothelium-independent vasodilator, shifted right Morphometrical analysis revealed that wall thickness cross-sectional area normalized wall:lumen ratio significantly aortas PARP-1 immunoreactivity confined level All these parameters unchanged Control-female. Conclusion Six PKD-male, not female, show dysfunction, smooth muscle contraction relaxation, pathological changes morphometry, apoptosis non-renal vasculature. The rat PKD males can identify novel targets treatment this disease.